Vaccination with immunodominant peptides encapsulated in Quil A-containing liposomes induces peptide-specific primary CD8+ cytotoxic T cells
Identifieur interne : 001D82 ( Main/Exploration ); précédent : 001D81; suivant : 001D83Vaccination with immunodominant peptides encapsulated in Quil A-containing liposomes induces peptide-specific primary CD8+ cytotoxic T cells
Auteurs : Grayson B. Lipford [Allemagne] ; Hermann Wagner [Allemagne] ; Klaus Heeg [Allemagne]Source :
- Vaccine [ 0264-410X ] ; 1994.
English descriptors
- Teeft :
- Adjuvant, Antigen processing, Cytotoxic, Effector, Epitope, Experimental points, Immune, Immune response, Immunodominant, Immunogenic, Immunol, Inoculation, Inoculum, Iscom, Iscoms, Lipford, Lipid, Liposome, Liposome structure, Listeriolysin, Lymphocyte, Lysis, Morein, Natl acad, Ovalbumin, Pathway, Peptide, Peptide vaccination, Quil, Quil liposomes, Ratio figure, Responder, Soluble proteins, Synthetic peptides, Target cells, Vaccination, Vaccine, Vivo, Vivo induction.
Abstract
Abstract: Immunostimulating complexes (ISCOMs), containing lipids, the saponin Quil A, and proteinaceous antigens, have been proven to vaccinate effectively CD8+ cytolytic T cells in vivo. However, conventional ISCOM technology is restricted to hydrophobic proteins or fatty acid-derivatized proteins or peptides. We therefore analysed whether Quil A-containing liposomes are an effective vehicle to shuttle hydrophilic proteins or peptides into the MHC class I pathway of antigen presentation resulting in the in vivo induction of antigen-specific cytolytic T cells (CTL). Liposomes were formed by a lipid dry-down method followed by resuspension with an aqueous solution containing protein/peptide and Quil A and then an extrusion step. Quil A-containing liposomes are an effective means to elicit a CD8+ CTL response to peptide antigen in vivo. CTL could be raised in C57B1/6 mice against ovalbumin (OVA) peptide 257–264 and vesicular stomatitis virus nucleoprotein 52–59, as well as in Balb/c mice against listeriolysin peptide 91–99 and cytomegalovirus pp89 168–176, demonstrating the versatility of this approach. The elicited response was peptide-specific, peptide dose-dependent and Quil A was necessary. Vaccination with liposomes entrapping the whole ovalbumin molecule or an extended (OVA) peptide 254–276 also yielded a CTL responsive to the immunodominant OVA peptide 256–264, implying cellular internalization and correct processing. Thus Quil A-containing liposomes appear to be a versatile vehicle to vaccinate CD8+ T cells in vivo; in addition, they could rapidly enhance the understanding of subunit vaccines and rules of antigen processing and peptide-MHC class 1 binding.
Url:
DOI: 10.1016/0264-410X(94)90013-2
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Abstract: Immunostimulating complexes (ISCOMs), containing lipids, the saponin Quil A, and proteinaceous antigens, have been proven to vaccinate effectively CD8+ cytolytic T cells in vivo. However, conventional ISCOM technology is restricted to hydrophobic proteins or fatty acid-derivatized proteins or peptides. We therefore analysed whether Quil A-containing liposomes are an effective vehicle to shuttle hydrophilic proteins or peptides into the MHC class I pathway of antigen presentation resulting in the in vivo induction of antigen-specific cytolytic T cells (CTL). Liposomes were formed by a lipid dry-down method followed by resuspension with an aqueous solution containing protein/peptide and Quil A and then an extrusion step. Quil A-containing liposomes are an effective means to elicit a CD8+ CTL response to peptide antigen in vivo. CTL could be raised in C57B1/6 mice against ovalbumin (OVA) peptide 257–264 and vesicular stomatitis virus nucleoprotein 52–59, as well as in Balb/c mice against listeriolysin peptide 91–99 and cytomegalovirus pp89 168–176, demonstrating the versatility of this approach. The elicited response was peptide-specific, peptide dose-dependent and Quil A was necessary. Vaccination with liposomes entrapping the whole ovalbumin molecule or an extended (OVA) peptide 254–276 also yielded a CTL responsive to the immunodominant OVA peptide 256–264, implying cellular internalization and correct processing. Thus Quil A-containing liposomes appear to be a versatile vehicle to vaccinate CD8+ T cells in vivo; in addition, they could rapidly enhance the understanding of subunit vaccines and rules of antigen processing and peptide-MHC class 1 binding.</div>
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